The expression of COX-2 and TRX-1 in breast cancer and the correlation with clinicopathologic characteristic and prognosis

Objective:To detect the expression of COX -2 and TRX - 1 using immunohistochemical SP methods to investigate the relationship between them,as well as with clinicopathological parameters,disease free survival time (DSF) in human breast cancer.Methods:Total of 122 breast cancer paraffin - embedded tissue was made into chip. These patients not only underwent completely resection but also accepted standard chemotheray or radiotherapy.Immunohistochemical staining SP method was performed.DFS ranged from the date of operation to relaps or follow - up (July,2008 ).There were 45 patients relaped.Results:The expression rate of COX - 2 and TRX -1 was higher (58.2%,54.1%) and the correlation between them was positive(r =0.286,P =0.001).Expression of COX -2 was positive correlated with tumour size(r =0.192,P =0.034),clinical stage(r =0.193,P= 0.033),lymph node metastasis (r =0.186,P =0.040).Expression of TRX-1 was positive correlated with tumour size(r =0.236,P =0.009) and clinical stage(r =0.206,P =0.023),but not correlated with lymph node metastasis(r =0.175,P= 0.054).Expression of COX - 2 or TRX -1 or COX - 2 and TRX - 1 was negative correlated with DFS by Kaplan - Meier survival analysis(P = 0.027,P = 0.046,P = 0.017 ).Conclision:Over - expression of COX - 2 and TRX - 1 associated with a poor clinical outcome.In breast cancer TRX -1 possibly regulates expression of COX -2.