Medium optimization for production of anti-streptococcal liamocins by Aureobasidium pullulans ☆

Abstract Liamocins are antimicrobial compounds with specificity for Streptococcus spp., produced by certain strains of the fungus Aureobasidium pullulans . Recent studies have identified strains and culture medium for liamocin production. However, this culture medium has not previously been optimized for liamocin production. In this study, we employ one-factor-at-a-time and statistical methods to develop an optimized medium for liamocin production. Liamocin yields from optimized medium were nearly double those from standard medium, up to 22 g liamocin/L. This represents the highest yields of liamocin reported to date. Furthermore, MALDI-TOF analyses show that liamocins produced from optimized medium are similar to those produced from standard medium.