Use of Peroxidase Isozyme in Testing the Genetic Purity of Some Micropropagated Plant in Sugarcane

Peroxidase isozyme was studied in the leaf tissue of four genotypes of Sugarcane viz BO91, BO110, BO120 and BO128 along with invitro cultured micropropagated plants using horizontal starch gel electrophoresis. Variation was observed among different genotypes with regard to number, position and intensity of bands. Micropropagated plant showed a pattern similar to their parental clones with regard to number and position of bands. Minor variation was observed for intensity of some bands among plants obtained throughin vitro culture. The plant regeneration through tissue culture were identical to their parental clone, indicating that during tissue culture process genetic purity of the genotype have been maintained and the protocol used in the present investigation is suitable for undertaking mass multiplication of clones through micropropagation. The isozymes of peroxidase can be used to distinguish the genotypes and monitor the genetic purity of plants obtained throughin vitro culture.