Antimicrobial Activity of Ethanol Extract from Leaves of Casearia sylvestris

AbstractCasearia sylvestris. Sw., also known as “guacatonga,” is a medicinal plant with broad use in South America. Among the popular applications attributed to this plant are anti-inflammatory, anticancer, antimicrobial, and antiulcer activities. Despite the broad popular use of this plant as a phytopharmaceutical agent, there are few studies about the antimicrobial potential of guacatonga. In this work, we have studied the antimicrobial potential of an ethanol extract obtained from C. sylvestris. leaves against three yeasts, two filamentous fungus, six Gram-negative bacteria, and two Gram-positive bacteria. Through two chromatographic steps using a Sephadex LH-20 column and RP-HPLC, we isolated and characterized two derived compounds of gallic acid: isobutyl gallate-3,5-dimethyl ether (IGDE) and methyl gallate-3,5-dimethyl ether (MGDE). Both compounds showed antimicrobial activity. IGDE was much more efficient than MGDE in inhibiting yeasts (Candida albicans, Candida tropicalis., and Candida guilliermon...