Fed-batch fermentation process for producing microorganism oil by using arrow root starch as raw material

2010 
The invention relates to production of microorganism oil, in particular to a fed-batch fermentation process for producing the microorganism oil by using arrow root starch as a raw material and belongs to the technical field of biochemical engineering. A carbon source and a nitrogen source are simultaneously supplemented into a fermentation medium in a thallus exponential phase; and the carbon source is supplemented into the fermentation medium when the thallus is in a stationary phase. The fed-batch fermentation process has the advantages of prolonging the growing phase of the thallus to obviously improve the biomass of the thallus, only providing the carbon source so as to synthesize a large amount of oil when the thallus is in the stationary phase and improving the content and the yieldof the microorganism oil. A feeding process designed by the invention can keep the pH of the fermentation medium stable in the process of fermentation, reduces the using amount of acid and base for regulating the pH and saves production cost. The feeding mode is simple and practical and is convenient to operate.
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