Liposome fusion with planar lipid membranes

: Potentiodynamic method was used for investigating bilayer lipid membrane (BLM)- liposomes (LS) interaction. BLM was formed from egg lecithin and its mixture with phosphatidylserine (PS); LS-from PS. It is shown that in the presence of calcium in the aqueous phase (1-10 mM) and charged phospholipids in both LS and BLM, fusion of LS with BLM is observed. The fusion event was registered by measuring the increase of negative charge density on BLM surface opposite to that facing the LS.