Synthesis and biological characterization of 1α,24,25-trihydroxy-2β-(3-hydroxypropoxy)vitamin D3 (24-hydroxylated ED-71)

Abstract 24-Hydroxylated derivatives were synthesized in 24( R ) and 24( S ) forms by the convergent method as analogs related to 1α,25-dihydroxy-2β-(3-hydroxypropoxy)vitamin D 3 . In the convergent synthesis, the A-ring fragment, synthesized from diethyl D-tartarate, and the C/D-ring fragments in 24( R ) and 24( S ) forms (vitamin D numbering), obtained from vitamin D 2 via the Inhoffen-Lythgoe diol, were coupled in moderate yields to give 1α,24( R ),25-trihydroxy-2β-(3-hydroxypropoxy)vitamin D 3 and 1α,24( S ),25-trihydroxy-2β-(3-hydroxypropoxy)vitamin D 3 . In preliminary biological evaluations, 24-hydroxylation of 1α,25-dihydroxy-2β-(3-hydroxypropoxy)vitamin D 3 caused weakened affinity to vitamin D binding protein in vitro and less calcemic activity in vivo compared to the parent compound. While the affinity to vitamin D receptor in 24 (R ) epimer was sustained, the affinity in 24( S ) epimer was less than that of the parent compound.