Reversed phase high performance liquid chromatography determination of most biogenic amines in serum

rapid, efficient and accurate reversed-phase liquid chromatographic method was adopted for simultaneous evaluation of most biogenic amines in serum. The biogenic amine was extracted from serum by using 0.5 M Perchloric acid and derivatives with dansyl chloride, efficient gradient method was applied using fast liquid chromatography ODS column (FLC) (50x4.6 mm I.D), 3μm particle size, the mobile phase solution consisted of 0.02 M ammonium acetate (solvent A), and acetonitrile (solvent B), flow rate 1mL/min, the elated biogenic amine-dansyl chloride derivatives were monitored by UV at 254nm. The detection limit for all biogenic amine standards ranged between 0.1-0.5 ng/ml. The adult normal values of 12 biogenic amines were evaluated in 30healthy males and 20 healthy female volunteers, the recovery of spiked serum with biogenic amine in this study were between the ranges of 72.2-104 %. The method was efficiently sparked with baseline resolution of 12 biogenic amine in less than 15 minutes. Therefore, this method is suitable for analysis of multiple biogenic amines in the clinical diagnosis and drug quality control.