Purification of murine monoclonal IgM antibody

This paper presents the purification of a monoclonal IgM antibody against human tumor associated antigen Lewis\|Y by ion exchange chromatography and gel filtration. Enzyme\|linked immunosorbent assay (ELISA) and SDS\|polyacrylamide gel electrophoresis (PAGE) were used to identify purified IgM antibody. In flow cytometry analysis, the purified IgM antibody recognizes human breast tumor cell line MCF\|7 which expresses Lewis\|Y antigen. This work presents a new way for the purification of murine monoclonal IgM antibody. 