High throughput, nanoliter quantitative PCR

The recent completion of the human genome sequence has increased the need for high throughput quantitative transcription analysis. Quantitative PCR is an alternative to microarrays for accurate and precise expression analysis with single transcript copy sensitivity. A review of current research in miniaturized, high throughput qPCR suggests this technique will soon be a viable option to hybridization microarrays for large-scale genetic analyses.