Application of a new technique in two-dimensional TLC separation of multicomponent mixtures

In this publication a new technique in planar chromatography is presented. The technique connects two-dimensional chromatography with multiple gradient development. It enables separation of multicomponent mixtures such as plant extracts with simultaneous qualitative identification of components. On the same plate less polar aglycones and more polar glycoside derivatives can be identified simultaneously. The new technique was applied to analysis of anthraquinone derivatives in Rhamnus frangulae cortex methanol extract.