Effect of treatment with oligosaccharide on pathogenesis-related protein and cell wall substances in sunflower leaves.

Oligosaccharide elicitor was tested for its ability to induce an increase of pathogenesis-related proteins (PRs) and cell wall substances in sunflower leaves by spray method.After treatment with oligosaccharide,β-1,3-glucanase and chitinase activities increased,the maximum values were 36.38% and 6.35% more than that in the control group,respectively.The content of lignin and hydroxyproline-rich glycoprotein(HRGP) enhanced,39.15% and 47.13% more than that in the control group,respectively.In another experiment sunflower leaves treated with oligosaccharide and inoculated with Puccinia helianthi Schw.were assayed,β-1,3-glucanase,chitinase and cell wall substances were induced,their production were higher than those in other treatments.The results showed that the increase levels of these two enzyme and cell wall substances in sunflower leaves treated with oligosaccharide play an important role in the induced resistance to sunflower rust.