A New Technique in Scanning Near Field Optical Microscopy Used for Investigations on the Biological Samples

We present a new technique based on apertureless near field microscopy using a femtosecond Ti:sapphire laser that is used for the investigations on biological samples. This technique is integrated in a multimodal microscopy system. The beam of a Ti:sapphire laser is focused by using the objective of an inverted microscope on the surface sample. Electric field of the laser beam oscillates in a parallel plan with the sample surface and electric dipoles created by the electric field emit a radiation that is modulated by the AFM tip. This radiation is collected by the objective of the s-SNOM module, placed above the sample being sent to the detector. The images shown a better good contrast compared to those in s-SNOM.