LC/MS를 이용한 식육중 잔류 벤지미다졸계 구충제 분석법 연구

Recently, mass spectrometry coupled with liquid chromatography(LC/MS) has been a preferred technique for determination of organic compounds in complex matrixes. LC/MS provides a high degree sensitivity and specificity of the compounds of interest. The purpose of this study was to confirm analytical method of residual 6 benzimidazoles (thiabendazole, oxfendazole, mebendazole, albendazole, flubendazole and fenbendazole) in meat by LC/MS. Benzimidazoles were analyzed by LC/MS on XTerra C18 column with 0.01% trifluoroacetic acid-acetonitrile(TFA) in a gradient mode as mobile phase, and that were identified by electrospray ionization with selected ion recording mode at 150-350amu mass range. Residual benzimidazoles were extracted from tissue with ethylacetate, and elute benzimidazoles with 50% acetonitrile. In the LC/MS analysis of benzimidazoles, signal to noise ratio was showed relatively high in the positive mode and special ion in the quality analysis was determined via [M+H]+ and Fragment ions. A spectrum of benzimidazoles was showed from all 6 benzimidazoles