DRSP: a Structural Database for Single Residue Substitutions in PDB

Substitutions of residues introduced by gene mutations can impact protein structures and often alternate protein properties. Identifying substitution-related structural changes is crucial for understanding their effects in biological functions. With the explosive numbers of experimentally determined protein structures, more and more wild-type-mutant structures have been used in comparable studies of structural biology. In this work, we constructed a structure-pair database, named DRSP, which collected non-redundant pairs of proteins with single residue substitutions in the entire Protein Data Bank. DRSP demonstrates abundant information for mutant-structure prediction and protein design. More importantly, DRSP presents the superimposed pairs of wild-type-mutant structures for backbone-perturbation studies, which are very important for the highly accurate structure modeling. DRSP is available at http:// www.labshare.cn/drsp/index.php .