Quantitative estimation of propranolol HCL and hydrochlorothiazide in pharmaceutical dosage form by micellar liquid chromatography

Micellar liquid chromatographic method has been developed for the simultaneous determination of Propranolol hydrochloride and Hydrochlorothiazide from bulk and formulations. Chromatographic separation achieved isocratically on ODS hypersil C18 column (5 μm, 250 mm × 4.6 mm) and micellar mobile phase of 0.07M sodium dodecyl sulfate (SDS) pH 3 adjusted with phosphate buffer and 15 % (v/v) 1- propanol as organic modifier in the ratio 70: 30 v/v and ultraviolet detection at 274 nm are used for the determination. In the developed method Propranolol hydrochloride and Hydrochlorothiazide elute at typical retention times of 6.767 min and 2.633 min, respectively at a 1 mL/min flow rate. Parameters such as linearity, precision, accuracy, specificity and robustness are studied as reported in the ICH guidelines. Linearity was observed in the concentration range of 4-24 μg/mL for Propranolol hydrochloride and and 2.5-15 μg/mL for Hydrochlorothiazide. The mean recoveries obtained for Propranolol hydrochloride and Hydrochlorothiazide were 99.33 %and 99.23 % respectively. Developed method was found to be accurate, precise, selective and rapid, can be applied routinely for the analysis of Propranolol hydrochloride and Hydrochlorothiazide in bulk as well as tablet dosage form.