Effect of blockage of the glucocorticoid receptor on the liver function of rats after ligation of the common hepatic artery

Objective To provide a theoretical basis for alleviating liver injury due to ischemia by improving the glucocorticoid receptor(GR) through observation of the relationship between liver injury due to ischemia and the pathway of the GR.Method A total of 208 SD rats were divided randomly into normal control(NC) group(n=8),simple ligation group(model group,n=40),blockage of 50% GR group(n=40),blockage of 80% GR group(n=40),blockage of 50% GR accompanied by ligation group(50% GR+M group,n=40),and blockage of 80% GR accompanied by ligation group(80% GR+M group,n=40).Ligation of common hepatic artery was adopted to reproduce a model of liver ischemia in rat,and to dynamically observe the changes in GR after the ligation of the common hepatic artery.The effects of blocking the GR on the liver functional parameters of rat serum [alanine aminotransferase(ALT),aspartate aminotransferase(AST),and alkaline phosphatase(ALP)],and the structure of liver tissue were observed.Results After the ligation of the common hepatic artery,peak values of serum ALT,AST,and ALP in the GR blockage group were higher than those of the simple ligation group.Furthermore,with increase in the degree of GR blockage,there was a tendency that the peak values of the enzyme value appeared earlier and the time of recovery to normal was delayed.In addition,the degree of inflammatory infiltration and ischemia necrosis of liver tissues were more severe than that in the simple ligation group.Immunohistochemistry revealed that after blockage of GR,GR in liver tissues was notably lower 2h after ligation than that in the simple ligation group,reaching its trough value at 6h.This phenomenon was more obvious in the 80% GR blockage group than that in the 50% GR blockage group.Conclusion The decrease of GR has an important effect on the course of liver ischemic injury.