Method for separating enramycin A from enramycin B

The invention discloses a method for separating enramycin A from enramycin B. The method comprises the following steps of: 1, dissolving a mixture of the enramycin A and the enramycin B; 2, soaking an octadecyl reversed phase bonded silica gel chromatography filling in methanol, and performing column packing; 3, loading a dissolution solution in the step 1 onto a reversed phase chromatography column, and eluting by using a sodium dihydrogen phosphate-phosphoric acid aqueous solution in an amount which is 4 to 6 times of the volume of the filling; 4, eluting the reversed phase chromatography column which contains the enramycin A and the enramycin B and is obtained in the step 3 by using the sodium dihydrogen phosphate-phosphoric acid aqueous solution containing 32 to 36 percent of methanol; 5, increasing the content of the methanol in the sodium dihydrogen phosphate-phosphoric acid aqueous solution step by step until the content is 40 percent, and performing gradient elution; and 6, eluting the reversed phase chromatography column treated in the step 4 by using the sodium dihydrogen phosphate-phosphoric acid aqueous solution containing 40 to 50 percent of methanol. By the method, the enramycin A can be separated from the enramycin B effectively.