Lipoid plasmid and preparation method of formulation thereof

The invention relates to lipoid plasmid and a preparation method of formulation thereof, and the lipoid plasmid is prepared by using an improved thin film dispersion-homogenization method, a freeze drying method/spray drying method. The prescription component is as follows: the mass ratio of drug and carrier is 1 to 68:100, the molar ratio of non-ionic surface active agent (HLB value is adjusted from 2 to 18) and cholesterol is 1:0.5 to 1.5, and the mass ratio of the drug and stabilizer (dicetyl phosphate) is 1:0 to 1.5. The preparation process comprises the preparations of blank lipoid plasmid, homogenized lipoid plasmid, drug-carrying lipoid plasmid and suspension thereof, and the operations of lipoid plasmid powder by freeze drying/spray drying. The lipoid plasmid of the invention has the abilities of preventing the drug from being swallowed by a hepatic tissue reticuloendothelial system, prolonging the residence time of drug in the body and assisting the drug to pass through blood brain barrier. In the invention, the lipoid plasmid has the characteristics of good stability and simple process, thus being applicable to large-scale industrial production.