Single nucleotide polymorphisms of follicle stimulating hormone receptor gene of Wenchang chicken

The objectives of the present study were to detect polymorphisms in the follicle stimulating hormone receptor(FSHR) gene,and to provide a scientific basis for marker assisted selection for high prolificacy in Wenchang chicken.In this study,the FSHR gene as the candidate gene of chicken's reproductive trait was analyzed on its exon1 to exon9 regions which encode most of the extracellular domain by PCR-SSCP.The result showed that 4 SNPs sites were found at exon 2 region,4 region,6 region,8 region respectively.They are C-49→T-49 mutation before the CDS' 5-flanking terminal of the exon2 region,T43→C43 mutation in the CDS of the exon4 region,A12→G12 mutation after the CDS' 3-flanking terminal of the exon6 region,G38→T38 mutation after the CDS' 3-flanking terminal of the exon8 region.The fitness test indicated the gene frequency were all in Hardy-Weinberg balanced state(P0.05).In conclusions,the polymorphisms of these sites lay a foundation for the further research on relationship between the polymorphisms of FSHR gene and the reproductive performance of Wenchang chicken.