Protective Effect of Polygonum orientale Flower Extract with Different Preparation Methods on Acute Myocardial Ischemia in Mongrel Dogs

OBJECTIVE: To observe the effect of Polygonum orientale flower extract with different preparation methods on acute myocardial ischemia in mongrel dogs. METHODS: The acute myocardial ischemia model of dog was set up though ligation of anterior descending branch of coronary artery in dogs, then the epicardium electrocardiogram was used to measure the degree of myocardial ischemia and the quantitative histological assay was adopted to determine the area of myocardial ischemia. At the same time, the lactic dehydrogense (LDH) and creatine kinase(CK) levels in serum were analyzed. All of them were used to evaluate the protective effect of Polygonum orientale flower with different preparation methods on acute myocardial ischemia in mongrel dog. RESULTS: Compared with the model group, the sample whose preparation methods was extracted by n-butanol after water extracting and alcohol precipitation showed obvious reduction in the area of myocardial ischemia,with decline in ∑ST and NST. Moreover,the LDH and CK levels in serum were decreased (P <0. 05 or P <0. 01). CONCLUSION: The sample which is extracted by n-butanol after water extracting and alcohol precipitation has a protective effect on injury induced by acute myocardial ischemia in mongrel dog.