Identification of Bacteria by Surface-Enhanced Raman Spectra after Peroxide Treatment

Surface-enhanced Raman spectroscopy (SERS) is a prospective method for the detection and identification of microorganisms. Hereby we describe the method for the registration of bacterial SERS spectra, including the mild oxidation sample treatment for the additional amplification of SERS signal. We also propose the spectral data processing method for the calculation of distinguishing criteria for the bacterial spectra.