Comparative genomic analysis of Leptospira borgpetersenii serogroup Javanica isolated from Rattus species in Southern Japan, Philippines, and Taiwan.

Abstract Leptospira spp. are the causative agents of leptospirosis, a zoonosis that occurs worldwide, and are maintained by various mammals. Recent molecular typing studies have indicated the existence of a specific Leptospira genotype-host association; however, few studies have shown the colonization of a single genotype in multiple animal genera. To gain further insights into the Leptospira genotype-host association, we conducted whole genome sequencing of 35 strains of L. borgpetersenii serogroup Javanica isolated from various Rattus species in Southern Japan, Philippines, and Taiwan. The SNP-based phylogenetic tree showed that the strains were grouped into five clusters based on their geographic location, indicating geographical structuring of genetic diversity in L. borgpetersenii serogroup Javanica strains. Furthermore, phylogenetic analysis indicated that the L. borgpetersenii serogroup Javanica can infect various animal species, thus making these strains generalist pathogens. This study reports the whole genome comparison of L. borgpetersenii serogroup Javanica strains isolated from Rattus species in East Asian states. The data herein suggest that the serogroup Javanica strains can infect the dominant Rattus species in each region and can be genetically diversified in a geographic manner.