Yam polysaccharide improves sperm viability and protects sperm DNA integrity in vitro
2017
Objective To clarify the roles of yam polysaccharide (YPS) in improving sperm viability and protecting sperm DNA integrity in vitro and provide a new approach to the treatment of oligoasthenozoospermia. Methods We collected samples by masturbation from 36 normal fertile males aged 27-39 years. Each sample was divided into six groups: blank control or treated with normal saline, vitamin C solution, and YPS solution at low (0.25 mg/ml), medium (1.0 mg/ml) or high concentration (5.0 mg/ml). Using eosin-Y staining, sperm hypotonic swelling (HOS) and sperm chromatin diffusion (SCD) test, we observed the effects of different concentrations of YPS on sperm viability, membrane integrity and nuclear DNA. Results After 24 and 48 hours of treatment, sperm viability was markedly reduced in the vitamin C ([28.5 ± 3.1] and [6.5 ± 1.2]%), low-YPS ([31.3 ± 3.5] and [6.5 ± 2.2]%), medium-YPS ([37.1 ± 3.5] and [9.5 ± 2.8]%) and high-YPS groups ([38.3 ± 3.3] and [9.0 ± 3.2]%) as compared with the blank control ([17.3 ± 2.1] and [3.2 ± 1.3]%) (P 0.05). Conclusions Yam polysaccharide can improve sperm viability and protect sperm DNA integrity in vitro.
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