Isolation of ubiquitinated substrates by tandem affinity purification of E3 ligase–polyubiquitin-binding domain fusions (ligase traps)
2016
This protocol describes the use of F-box–UBA domain fusion proteins known as ligase traps to capture and enrich for ubiquitinated proteins. By following a two-step purification procedure, enriched substrates can be identified by mass spectrometry.
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