Influence of colonic dialysis using Chinese medicine on creatinine decomposition by intestinal bacteria in uremia rats.

OBJECTIVES: This study aims to investigate influence of colonic dialysis using Chinese medicine on creatinine decomposition by intestinal bacteria in uremia rats. METHODS: Healthy male Sprague Dawley (SD) rats were randomly divided into three groups, including uremic group, sham group and Chinese medicinal group. Uremic model was established in uremic group and Chinese medicinal groupby 5/6 nephrectomy, while sham group did not undergo nephrectomy. All rats were sacrificed at the end of ten weeks. Serum creatinine was examined. Histopathological changes of rat kidney were observed by hematoxylin-eosin staining. The numbers of Bifidobacterium, Lactobacillus and E.coli in the intestinal tract were quantitatively determined by real-time fluorescent quantitative PCR with 16S RNA. RESULTS: Compared with sham group, the number of E.coli in the jejunum increased significantly, while that of Bifidobacterium and Lactobaeillus in the ileum decreased. The number of Bifidobacterium and Lactobaeillus was decreased in the colon whereas that of E.coli increased. CONCLUSION: Our findings revealed that Influence of colonic dialysis using Chinese medicine on creatinine decomposition by intestinal bacteria in uremia rats.