Simultaneous analyte indicator binding assay (SBA) for the monitoring of reversible host-guest complexation kinetics.

Very few information is available for the kinetics of the self-assembly and dissociation of optically silent building blocks despite the importance of such data in the rational design of tailor-made host-guest systems. We introduce here a novel time-resolved method that enables the simultaneous determination of complex formation and complex dissociation rate constants for inclusion-type host-guest complexes. Noteworthy, the simultaneous analyte indicator binding assay (SBA) gives also direct access to binding affinities, thus largely simplifying the experimental procudure for a full kinetic and thermodynamic characterisation of host-guest systems.