A kind of method of multiple water-soluble vitamin in blood sample of detection simultaneously

The invention discloses a kind of method of multiple water-soluble vitamin in blood sample of detection simultaneously, first pass through simple liquid-liquid extraction method and biological specimen is carried out pre-treatment, subsequently into chromatographic isolation and Mass Spectrometer Method, a pair qualitative ion and a pair quota ion is selected respectively for every kind of vitamin, using the relative retention time of various vitamin and qualitative ion pair as qualitative foundation, make standard curve with standard substance quantitative.Meanwhile, the quality-control product of three levels of this method application investigates the accuracy of method, effectiveness, it is to avoid testing result distortion.The present invention achieves the purpose that nine kinds of water soluble vitamins in a serum sample are detected by application LC-MS technology simultaneously first, reduce chaff interference impact, the method fast analysis time easy and simple to handle only 5min, flux is high, cost is low, effectively water soluble vitamins level in monitoring human body, reasonable, the safety of vitamin are supplemented there is directive significance, it is easy to clinical expansion and universal.