Method for detecting tissue cell protein by using housekeeping protein

The invention relates to a method for detecting tissue cell protein by using housekeeping protein. According to the method, housekeeping protein and a monoclonal antibody or a polyclone antibody of protein to be detected are coupled with colloidal gold or colloidal silver respectively, the colloidal gold or silver antibody compound formed after coupling is dripped on a gold marking pad of a test paper; and another monoclonal antibody or polyclone antibody against corresponding housekeeping protein or protein to be detected is fixed at a detection area of the test paper, and the negative or the positive of the protein detected is judged according to the relative calculation ratio of the color of the housekeeping protein on the test paper to the color of the detected protein. The detection method solves the quantitative problem in colloidal gold immunoassay, and widens the application of colloidal gold immunoassay in protein detection; and in addition, the detection method has the advantages of fastness in speed, high accuracy and the like compared with other protein detection.