Construction of eukaryotic recombinant sTRAIL expression vector and study on its anti-C6-glioma activity in vitro

Objective To construct eukaryotic recombinant sTRAIL expression vector and investgate its activity against C6 glioma cell line.Methods After construction of eukaryotic recombinant sTRAIL expression vector--PcDNA -sTRAIL by means of PCR and directed cloning,the C6 glioma cell line was transfected by cationic liposome and the proliferation and apoptosis of cells was detected by FCM.Results The construction of eukaryotic recombinant sTRAIL expression vector--PcDNA-sTRAIL confirmed a success by gene sequencing and western blot.After transfected by cationic liposome,50 percents of C6 cells expressed possitive protein.The apoptosis rate of C6 cell was significant higher in PcDNA-sTRAIL plus Lipofectin transfected group than that in three control groups(t=9.52,7.20,2.84 respectively,P0.05).Conclutions An eukaryotic recombinant sTRAIL expression vector was successfully constructed and its anti-C6-glioma activity was confirmed in vitro study.