Euro Clinical Microbiology 2020 Isolation, molecular identification and genomic pattern of Mycobacterium bovis isolates collected from tuberculin-positive cattle in infected farms of Shiraz

Mycobacterium bovis is the main cause of tuberculo-sis in cattle. At global scale and also in Iran, the most frequent currently-in-use method in detection of in-fected cattle is tuberculination.The present study was aimed to improve our genom-ic knowledge of Mycobacterium bovis population structure in cattle farms of Shiraz.Fifty pathological samples from tuberculin-positive cattle collected from two slaughterhouses at Shiraz were subjected to bacterial culture on glycerinated and pyruvated Lowenstein-Jensen media. Genomic material from culture-positive slopes was extracted and used in PCR-16S rRNA, PCR-IS6110, and PCR-RD typing experiments. All the M. bovis isolates were further genotyped using pvu II-digested PGRS-RFLP strategy.