Determination of astragaloside IV in the hairy root cultures of membranous milkvetch (Astragalus membranaceus) by RP-HPLC

An accurate HPLC method for the determination of astragaloside IV in the hairy root cultures of Astragalus membranaceus (Fisch.) Bunge was developed. Calibration graphs was rectilinear between 10.64 mug and 53.20 mug under the experimental conditions. The recovery was 92.66%. The relative standard deviations (RSDs) of measurement precision test were 4.20% and 2.40%. The astragaloside IV content measured in the hairy root cultures was 0.14% (dry weight).