Using Realtime Rt-Pcr and Sequencing Assays to Define Viral Load, Types and Subtypes of Hepatitis C Virus at Cantho Center General Hospital

We used Realtime RT-PCR assay to count the viral load on 94 patients who having positive anti-HCV, to find the types of HCV on 85 patients with positive HCV RNA. Then, we chose 6 samples to sequence to figure out the subtypes of HCV. The first result showed that: most of patients had the viral load ≤ 2x106 copies/ml (92.5%). The types of HCV have been found: type 1 (47.1%), type 6 (25.9%), and type 2 (21.6%). Moreover, there was 5.8% the co-infection between type 1 and type 6 on the patients. There was the similar result between Realtime RT-PCR assay and sequencing technique on typing HCV. We have been known the subtypes of HCV by sequencing technique, they are 1b, 2c, 6a, 6e and co-infection 1b/6e.