ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATIO N OF CARBIDOPA, LEVODOPA AND ENTACAPONE IN ITS BULK AND TABLET DOSAGE FORM BY UPLC

The study was conducted with the purpose to method develop and validate a Ultra Performance Liquid Chromatography m ethod UV detector to determine Levodopa, Carbidopa and Entacapone content in table preparation using MeOH : Acetonitrile : phosphate buffer (pH 2.8, 30:40:3 0 v/v/v). Column is BEH C 18 (2.1 x 100 mm, 5 μ m), flow rate 0.3 ml per min ute and run time is 4 min u tes . The method was validated toward parameters of accuracy, precision, linearity, LOD, LOQ, flow rate, robustness of the Levodopa, Carbidopa, and Entacapone tablet in the form of LOD respectively 0.0060, 0.0 064, and 0.35 and LOQ respectively 1.30, 1.44 an d 1.96