[IFN-gamma promotes human monocytes to differentiate to untypical mature DC].

AIM:To explore the morphologic and phenotypic changes of monocytess in response to the stimulation of IFN-γ,TNF-α and IFN-α.METHODS:Newly isolated monocytes from human PBMCs were treated with IFN-γ,TNF-α or IFN-α.The phenotypic growth of monocytes with or without stimulation was observed by microscope.The surface expression of CD1a,CD14,CD80,CD83,CD86,and HLA-DR on the monocytes was assayed by flow cytometry.RESULTS:After IFN-γ stimulation,monocytes displayed the shape of fusiform or polygon and were more likely to adhere and cluster to form the distinct structure as "cell islet".IFN-γ also induced or up-regulated the surface expression of CD80,CD83,CD86,and HLA-DR on the monocytes and down-regulated the CD14 expression.IFN-γ even induced the CD1a expression and changed the monocytes phenotype from CD14+CD1a-CD83-to CD14+CD1a+CD83+ after stimulated for 5 days.Cytokines TNF-α and IFN-α have the different impacts on monocytes from IFN-γ.CONCLUSION:IFN-γ have the immunoregulatory function of making monocytes differentiate to untypical mature dendritic cells(DC).