Effect of nuclear factor-kappa B on parathyroid hormone receptor expression and hypocalcaemia in rats with acute necrotizing pancreatitis

Objective To investigate the effect of nuclear factor-kappa B（NF-kB） on the expression of parathyroid hormone receptor （PTH1R） and serum calcium level in rats with acute necrotizing pancreatitis （ANP） rats. Methods 24 male Sprague-Dawley rats were randomly divided into sham operation group （SO）, ANP group, pyrrolidine dithiocarbamat （PDTC, an inhibitor of NF-kB） pretreated ANP group （PDTC）, with 8 rats each group. ANP model was induced by injecting 5% sodium taurodeoxycholate into biliary pancreatic duct. Serum calcium was measured and the expression of NF-kB was determined by western blot and the expression of PTH1R was examined by RT-PCR plus western blot 6 h after ANP induction. Results The NF-KB proteins of bone and kidney tissues in ANP group were 1.53 ± 0.08 and 0.47 ± 0.19, respectively; in PDTC group they were 0.61 ±0.13 and 0.36 ± 0.06 with significant difference （ P 〈 0.05 ）. The PTH1R mRNA expressions of bone and kidney in ANP group were 0. 27 ±0.08 and 0.29 ± 0. 06, respectively; the PTH1R proteins expressions were 0. 87 ± 0. 07 and 0. 31±0. 09, respectively. The corresponding values in PDTC group were 0.57 ± 0.27 and 0.37 ± 0. 11, 1.13± 0. 17 and 0.68 ± 0.15, respectively, with significant difference （P 〈 0.05 ）. The activity of NF-KB increased in ANP group, the expressions of PTH1R decreased significantly. Compared with ANP group, the activity of NF-kB decreased and the levels of PTH1R and serum calcium increased in PDTC group （ P 〈 0.05 ）. Conclusions NF-kB may indirectly down-regulate the expression of PTH1R, which resulting into the hypocalcaemia of ANP. PDTC may alleviate hypocalcaemia in ANP. Key words: Pancreatitis, acute necrotizing;  NF-kB;  Receptor, parathyroid hormone;  Hypocalcaemia