Fine mapping of a cdt locus mutation that leads to an increase in the tolerance of pea (Pisum sativum L.) to cadmium

The SGECdt pea mutant (cdt), which has an increased level of cadmium accumulation and tolerance to cadmium as compared to the initial line, was recently created. Earlier, a sequence specific amplified polymorphism analysis detected the location of the cdt locus in pea linkage group VI. For mapping of the cdt locus in more detail, a set of molecular markers that was based on the known sequences of pea genes was developed. The sequences were determined using the analysis of genome microsynteny between pea and the model legume Medicago truncatula. A close linkage between the cdt locus and the markers elaborated on the base of Pentatricopeptide repeat and Exosome complex exsonuclease RRP45 genes was revealed. Thus, the prerequisites for further cdt positional cloning were developed.