Effect of split doses of N-methyl-N-nitrosourea on DNA repair synthesis in cultured mammalian cells.

Summary DNA repair was measured in human fibroblasts, mouse C3H 10 T 1/2 fibroblasts and rat hepatocytes by the non-semi-conservative incorporation of [ 3 H]TdR during DNA repair synthesis using liquid scintillation techniques. Confluent monolayers of these cells grown on cover slips were exposed to split doses (125 or 250 μg/ml) of the mutagenic and carcinogenic alkylating agent MNU and DNA repair synthesis compared with that produced by a single dose (500 μg/ml). No significant difference in DNA repair capacity was detected in the three cell lines treated with a single dose or split doses of MNU.