Kinetic changes of lactate dehydrogenase in hepatopancreas and abdominal muscle of the crayfish orconectes limosus raf. (crustacea: decapoda) after eyestalk removal

Abstract 1. 1. LDH kinetic studies of hepatopancreas and abdominal muscle in Orconectes limosus Raf. crayfish have demonstrated a kinetic resemblance of this enzyme in both tiflaes to the “muscle” type LDH of vertebrates. 2. 2. Decrease of K m (PA) and K m (LA) values as well as an increase of enzyme activity inhibition by higher pyruvate concentration after eyestalk removal was observed. This indicates the LDH kinetic change of both examinated crayfish tiflaes in the direction characteristic for LDH of “heart” type in vertebrates. 3. 3. Results obtained in this work suggest a LDH adaptation of both investigated tiflaes to the changed respiratory metabolism in crustaceans after eyestalk removal.