Genomic DNA extraction and PCR amplication of haptophytes

Phaeocystis globosa and Pavlova viridis which are colony and single cell representative were used as samples.Genomic DNA extraction from those haptophytes was studied.4 methods for DNA extraction were examined,based on PCR amplification of psbA gene.The results showed that both salt-extraction of high concentration and PVP-extraction were safe,quick and economical methods.The high-yield DNA was extracted by the two methods,but impurity.The method of CTAB-extraction was also safe and quick,but the yield of genomic DNA was low.The glass bead-extraction can obtain high-yield and purity genomic DNA,but process is complex and expensive.For P.globosa,the method of glass bead-extraction was the best one among 4 methods.For P.viridis,considered economical and convenient factors,the method of salt-extraction of high was the best.