A single nucleotide in the promoter region modulates the expression of the β-lactamase OXA-61 in Campylobacter jejuni
2014
Methods: An ampicillin-resistant derivative of C. jejuni NCTC 11168 was subjected to whole genome sequencing and comparative genomics analysis. Complementary molecularexperiments were further performed toexamine the identified regulatory mechanism of blaOXA-61, which included complementation, promoter fusion assay, realtime RT‐PCR, natural transformation using a defined PCR fragment, survey of clinical isolates and transcription start site mapping.
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