Evaluación toxicológica de la L-Glutamina

Se ha estudiado las propiedades antidrepanocitica de la L-glutamina como complemento en el tratamiento de la anemia drepanocitica, enfermedad muy frecuente en el mundo para la que aun no existe tratamiento efectivo. En el presente trabajo se realizo un estudio de actividad hemolitica de la L-Glutamina sobre eritrocitos normales y SS mediante la determinacion del por ciento de hemolisis a relaciones molares hemoglobina: L-glutamina (1:1, 1:4, 1:8 y 1:10) empleando una tecnica espectrofotometrica; se realizo ademas un test de clasificacion toxicologica segun la guia de la Organizacion para el Desarrollo y cooperacion Economica (OECD) a la dosis de 2000 mg/kg en ratas wistar (3 hembras y 3 machos); se les administro el vehiculo a otro grupo empleado como control. Se busco la presencia o no de sintomas y signos clinicos, las variaciones de peso de los animales antes y despues del tratamiento y se realizo la necropsia al finalizar el test. En el estudio de la actividad hemolitica no se encontraron diferencias significativas (p The antidrepanocytic properties of L-glutamine as a complement in the treatment of drepanocytic anemia, a very common disease in the world for which there is no effective treatment yet, were studied. A study of the hemolytic activity of L- Glutamine on normal erythrocytes and SS was conducted by the determination of the percent of hemolysis at molar ratios of haemoglobin to L-glutamine (1:1, 1:4, 1:8 and 1:10 ) by using a spectrophotometric technique. A test of toxicological classification was also made according to the guide of the Organization for Economic Cooperation and Development (OECD) at a dose of 2000 mg/kg in Wistar rats (3 females and 3 males). The vehicle was administered to other group used as control. The presence or not of symptoms, clinical signs, and variations of weight of the animals before and after treatment were searched. Necropsy was performed at the end of the test. In the study of hemolytic activity, no significant differences (p