Sample Preparation for High Throughput Screening of Complex Biomolecules Using a Multidimensional Approach

5000 4000 4 00 Mass (mIz) 300 HPLC and/or MALDI-TOF. Materials and Methods: 96-well min i-chromatography columns were prepared following the manufacturer's instructions by loading dry media into MultiScreen Resist O.4mm filter plates (MAR4N0410 , Millipore Corporation , Bedford MA) using eithe r a 25pl or a 45pl MultiScreen Column Loader (Millipore Corporation , Bedford MA). Media used included reverse phase resins of a) 300 A6-10 pm C18 (Waters-Microbondapak, Waters Corporation, Milford, MA); b) 300 A l Surn or 40 pm C4 (Amicon-Matrex, Millipore Corporation, Bedford, MA)j or c) R2 styrene-divinylbenzene(DVB), C4 like (PE-PerSeptive Biosystems,