Analysis of multiple data sets reveals no association between the insulin gene variable number tandem repeat element and polycystic ovary syndrome or related traits.

Context: Variation at the insulin gene VNTR (variable number tandem repeat) minisatellite has been reported to be associated with polycystic ovary syndrome (PCOS), but findings have been inconsistent and all studies have featured small sample sizes. Objective: To gain a robust understanding of the role of the INS-VNTR in PCOS susceptibility. Design: Case-control, family-based association and quantitative trait analyses. Setting and Participants: A UK population comprising 255 parent-offspring trios, 185 additional cases, and 1062 control subjects (cases and controls all British/Irish) as well as 1599 women from a northern Finland population-based birth cohort characterized for PCO symptomatology and testosterone levels. VNTR class was inferred from genotyping of the −23HphI variant. Intervention(s): None. Main Outcome Measure(s): INS-VNTR genotype frequencies between subject groups, body mass index, and testosterone levels by genotype. Results: Case-control analyses in both UK and Finnish samples failed t...