[Protective effect of ischemic preconditioning on ischemia-reperfusion injury].

Objective: To study the protective effect of ischemic preconditioning(IP) on ischemia reperfusion injury of limb. Methods: One to three times of short term ischemia was applicated before prolonged ischemia, a series of biological examinations, including creatine phosphate kinase(CPK), glutamic oxalacetic transaminase(GOT), lactic dehydrogenase(LDH), malondiadehyde(MDA) and superoxide dismutase(SOD) were carried out in each group. Results: Compared with the control, release of skeletal muscle intracellular enzyme(CPK, GOT and LDH) decreased in experiment groups after preconditioning had been performed. The serum concentration of MDA was lower, activity of SOD was higher, and pathological lesion was less in preconditioning groups than that in non preconditioning group. Different frequency of preconditioning led to different results. Conclusions: Preconditioning has protective effect on ischemia reperfusion injury of limbs, and the effect of IP is correlated with the number of preconditioning cycles. [