Trace Element Mapping of a Biological Specimen by a Full‐Field X‐ray Fluorescence Imaging Microscope with a Wolter Mirror

A full‐field X‐ray fluorescence imaging microscope with a Wolter mirror was applied to the element mapping of alfalfa seeds. The X‐ray fluorescence microscope was built at the Photon Factory BL3C2 (KEK). X‐ray fluorescence images of several growing stages of the alfalfa seeds were obtained. X‐ray fluorescence energy spectra were measured with either a solid state detector or a CCD photon counting method. The element distributions of iron and zinc which were included in the seeds were obtained using a photon counting method.