The effect of Pulsatillae combined with Endostar on the proliferation,migration,build of tube of vessel endothelial cells

Objective:To study the effect of pulstatissal combined with Endostar on the proliferation,migration,build of tube of vessel endothelial cells.Methods:Proliferations of human umbilical vein endothelial cells(HUVEC) were measured by MTT colorimetric assay treated by Pulsatillae combined with Endostar in vitro compared with human colon carcinoma Lovo cell.Using transwell cabin test,canaliculization test to observe the effect of Pulsatillae combined with Endostar on the migration,vaso-formed ability.Results: The survival rate of HUVEC was 37.6%-22.5% within the dose of 2-8μg/ml and negative correlated with the concentration(r=-0.850,P=0.032) treated with the alcohol extract of Pulsatillae.The survival rate of Lovo cell was more than HUVEC,so the alcohol extract of Pulsatillae had differential cytotoxicity to Lovo cell and HUVEC(P=0.000).And there was a synergistic effect when combined with Endostar 10μg/ml.The migration of HUVEC was obviously depressed treated with the alcohol extract of Pulsatillae at the doe of 2-8μg/ml combined with Endostar 10μg/ml in 12 hours,and negatively correlated with the concentration(r=-0.919,P=0.000).The number of tubule was reduced and lumina become unintegrated,there was significant difference compared with the control group treated with Pulsatillae 2-8μg/ml combined with Endostar 10μg/ml,and negatively correlated with the concentration(r=-0.908,P=0.006).Conclusion: Low dose Pulsatillae can actively suppress angiogenesis in vitro,and has synergistic effect with Endostar.