Plant regeneration from protoplasts of sugarcane

Calli were induced from young leaf explant of sugarcane(Saccharum of ficinarum L.). Through several subcultures embryogenic calli were selected and transfered to liquid MS_(3) medium for suspensive culture. When small round cells rich in cytoplasm developed in the suspension cultures, they could be selected for protoplast isolation. Protoplasts were cultured in MRP_(1) agarose medium containing sucrose. Protoplast-regenerated calli were of two merphological types. Those compact, granular calli were selected for different ation culture on N6 medium. When placed on N_(6) medium containing 0.5 mg/L KT, protoplasts-derived calli from ROC_(1) ROC_(1) regenerated green shoots and eventually grew in to a whole plant. However, only albino plants grew from G.D.57-423 and roots developed from US66-56-9 when they were placed on N_(6) medium supplemented with 0.1% activated charcoal with no growth regulato.