Epidemiological survey of bovine tuberculosis in Guangxi using interferon gamma ELISA detection

【Objective】Epidemic survey was carried out to find out the prevalence of bovine tuberculosis in Guangxi.【Method】The tuberculin skin test (TST) and interferon gamma (IFN-g) ELISA assay were used to detect the bovine tuber-culosis in Guangxi from 2008 to 2011. Totally 5478 cows and 1580 buffaloes had been quarantined for 4 years. 【Result】Dur-ing 2008-2011, 63 dairy cows (accounted for 1.15% of the total ) and 17 buffaloes(accounted for 1.08% of the total)were detected as positive in Nanning, Liuzhou, Beihai, Qinzhou and Guilin. Overall, the positive rate of tuberculosis in cow and buffalo decreased year by year. Through self-made box of interferon gamma assay and BovigamTMkit, it was found that the positive rate of bovine tuberculosis detected by interferon gamma assay which had high accuracy was lower than that by TST.【Conclusion】For better detection and purification of bovine tuberculosis, the herd should be quarantined by tuberculin skin test, and then tested by interferon gamma assay.