A temperature-jump NMR probe setup using rf heating optimized for the analysis of temperature-induced biomacromolecular kinetic processes

Abstract A novel temperature jump (T-jump) probe operational at B 0 fields of 600 MHz (14.1 Tesla) with an integrated cage radio-frequency (rf) coil for rapid ( 1 H-, 2 H- and 15 N-nuclei. High B 0 field homogeneities (0.7 Hz at 600 MHz) are combined with high heating rates (20–25 K/s) and only small temperature gradients ( 15 N-selectively labeled 40nt hsp17-RNA thermometer.